Ekologiya cheloveka (Human Ecology)Ekologiya cheloveka (Human Ecology)1728-08692949-1444Eco-Vector10990910.17816/humeco109909Research ArticleInfluence of individual and subpopulation factors on the concentration of major and trace elements in salivaSavinovSergey S.<p>Cand. Sci. (Chem.)</p>s.s.savinov@spbu.ruhttps://orcid.org/0000-0002-7214-5917DrobyshevAnatoly I.<p>Dr. Sci. (Phys.-Math.), Professor</p>drobyshevai@mail.ruhttps://orcid.org/0000-0002-9174-2055Saint Petersburg State University0512202229106896981608202206102022Copyright © 2022, Eco-Vector2022<p><strong><em>BACKGROUND:</em></strong> The determination of the major and trace element composition of saliva could be a promising approach for non-invasive health monitoring. Such investigations are constrained, nevertheless, by the potential impact of several variables on the concentration of the elements.</p>
<p><strong><em>AIM:</em></strong> To study the influence of individual and subpopulation factors of donors on the concentration of some major and trace elements in saliva.</p>
<p><strong><em>MATERIALS AND METHODS:</em></strong> The results of the determination of elements concentration (Ag, Al, Ca, Cd, Cr, Cu, Fe, Mg, Mn, P, Pb, Si, Ti, Zn) in the whole saliva of 60 donors by direct (without digestion) analysis by arc atomic emission spectrometry using the dry residue technique are presented.</p>
<p><strong><em>RESULTS:</em></strong> Distribution of concentrations in the sample for Al, Cr, Cu, Fe, Mg, Mn, Si, Ti, and Zn showed a lognormal law; thus, the geometric mean or median is preferable as an indicator reflecting the average value. The distribution of Ca and P concentrations obeys the normal law. The concentration of Si and Ti is lower in the saliva of young men compared with women; the opposite pattern is observed for Mn. The mean Ag concentration in saliva is higher in the group of smokers than in non-smokers. Increase in the number of teeth affected by caries was correlated with an increase in concentrations of Cu, Al, and Zn in the saliva. For several analytes, interelement correlations were revealed, in particular, between the concentrations of Ca and P.</p>
<p><strong><em>CONCLUSION:</em></strong> The study of such factors of influence enables clinicians to determine the normal concentration ranges of major and trace elements in each specific clinical case with greater accuracy.</p>salivatrace elementsmajor elementssubpopulation factorsaverage concentrationsgenderdental fillingsслюнамикроэлементымакроэлементысубпопуляционные факторысредние концентрацииполпломбы в зубах[Avcyn AP, Zhavoronkov AA, Rish MA, Strochkova LS. Mikrojelementozy cheloveka. Jetiologija, klassifikacija, organopatologija. Moscow: Medicina; 1991. 496 p. (In Russ).][Jacobs N, Nicolson NA, Derom C, et al. Electronic monitoring of salivary cortisol sampling compliance in daily life. Life Sci. 2005;76(21):2431–2443. doi: 10.1016/j.lfs.2004.10.045][Wang D, Du X, Zheng W. Alteration of saliva and serum concentrations of manganese, copper, zinc, cadmium and lead among career welders. Toxicol Lett. 2008;176(1):40–47. doi: 10.1016/j.toxlet.2007.10.003][Gil F, Hernandez AF, Marquez C, et al. Biomonitorization of cadmium, chromium, manganese, nickel and lead in whole blood, urine, axillary hair and saliva in an occupationally exposed population. Sci Total Environ. 2011;409(6):1172–1180. doi: 10.1016/j.scitotenv.2010.11.033][Fakour H, Esmaili-Sari A, Zayeri F. Scalp hair and saliva as biomarkers in determination of mercury levels in Iranian women: amalgam as a determinant of exposure. J Hazard Mater. 2010;177(1-3):109–113. doi: 10.1016/j.jhazmat.2009.12.002][Zahir S, Sarkar S. Study of trace elements in mixed saliva of caries free and caries active children. J Indian Soc Pedod Prev Dent. 2006;24(1)27–29. doi: 10.4103/0970-4388.22832][Watanabe K, Tanaka T, Shigemi T, et al. Mn and Cu concentrations in mixed saliva of elementary school children in relation to sex, age, and dental caries. J Trace Elem Med Biol. 2009;23(2):93–99. doi: 10.1016/j.jtemb.2009.01.003][Monaci F, Bargagli E, Bravi F, Rottoli P. Concentrations of major elements and mercury in unstimulated human saliva. Biol Trace Elem Res. 2002;89(3):193–203. doi: 10.1385/BTER:89:3:193][Watanabe M, Asatsuma M, Ikui A, et al. Measurements of several metallic elements and matrix metalloproteinases (MMPs) in saliva from patients with taste disorder. Chem Senses. 2005;30(2):121–125. doi: 10.1093/chemse/bji007][Kostyuchek DF, Klyukovina AS, Lebedeva TV. Magnesium's contents in the saliva and hair of the cervix elongation's patients. Journal of Obstetrics and Womans Diseases. 2006;55(3):45–48. (In Russ).][Costa de Almeida GR, de Freitas CU, Barbosa FJr, et al. Lead in saliva from lead-exposed and unexposed children. Sci Total Environ. 2009;407(5):1547–1550. doi: 10.1016/j.scitotenv.2008.10.058][Notova SV, Ordzhonikidze GZ, Nigmatullina JuF. Soderzhanie himicheskih jelementov v sljune i volosah detej, prozhivajushhih v rajonnom centre Saraktash Orenburgskoj oblasti. Vestnik Orenburgskogo Gosudarstvennogo Universiteta. 2003;6:146–147. (In Russ).][Esteban M, Castano A. Non-invasive matrices in human biomonitoring: a review. Environ Int. 2009;35(2):438–449. doi: 10.1016/j.envint.2008.09.003][Drobyshev AI, Ryadchikova NA, Savinov SS. Atomic emission analysis of human hair for the presence of trace elements. Journal of Analytical Chemistry. 2016;71(7)717–722. (In Russ). doi: 10.1134/S1061934816070078][Savinov SS, Sharypova RM, Drobyshev AI. Determination of the trace element composition of human nails. Journal of Analytical Chemistry. 2020;75(3):409–415. (In Russ). doi: 10.1134/S1061934820030168][Savinov SS, Drobyshev AI. Determination of trace elements in biological fluids by arc atomic emission spectrometry. Journal of Analytical Chemistry. 2022;77(3):328–333. (In Russ). doi: 10.1134/S1061934822010129][Drobyshev AI, Savinov SS. An experimental study of the luminosity of an mfc−maes-based digital spectrograph. Optics and Spectroscopy. 2016;120(2):335–338. (In Russ). doi: 10.1134/S0030400X16020077][Drobyshev AI, Savinov SS. Experimental study of the spread function and resolving power of an optical digital spectrograph based on an MFS polychromator. Journal of Optical Technology. 2014;81(1):33–38. (In Russ). doi: 10.1364/JOT.81.000033][Labusov VA, Garanin VG, Shelpakova IR. Multichannel analyzers of atomic emission spectra: current state and analytical potentials. Journal of Analytical Chemistry. 2012;67(7):632–641. (In Russ). doi: 10.1134/S1061934812070040][Drobyshev AI, Savinov SS. On certain features of spectrum recording and photometric measurements of spectral lines using a MFS–MAES-based digital spectrograph. Instruments and Experimental Techniques. 2013;56(6):693–696. (In Russ). doi: 10.1134/S0020441213050138][Savinov SS, Anisimov AA. Effect of conditions for sampling of human saliva on the results of determination of macro- and micronutrients. Journal of Analytical Chemistry. 2020;75(4):453–458. (In Russ). doi: 10.1134/S1061934820040139][Rodinkov OV, Bokach NA, Bulatov AV. Osnovy fiziko-himicheskih izmerenij i himicheskogo analiza. Saint Petersburg: VVM; 2010. 132 p. (In Russ).][Baranovskaya IA. The role of micro-elements in the inflammatory diseases of paradentium in chronic gastroduodenitis in children of school age. Kazan medical journal. 2009;90(1):87–89. (In Russ).][Aizenbud D, Peri-Front Y, Nagler RM. Salivary analysis and antioxidants in cleft lip and palate children. Arch Oral Biol. 2008;53(6):517–522. doi: 10.1016/j.archoralbio.2007.12.006][Garhammer P, Hiller KA, Reitinger T, Schmalz G. Metal content of saliva of patients with and without metal restorations. Clin Oral Investig. 2004;8(4):238–242. doi: 10.1007/s00784-004-0281-4][Abraham JA, Sanchez HJ, Grenon MS, Perez CA. TXRF analysis of metals in oral fluids of patients with dental implants. X-Ray Spectrometry. 2014;43(4):193–197. doi: 10.1002/xrs.2538][Sheibaninia A. The effect of social stress on salivary trace elements. Biol Trace Elem Res. 2014;162(1-3):58–63. doi: 10.1007/s12011-014-0119-0][Wilhelm M, Pesch A, Rostek U, et al. Concentrations of lead in blood, hair and saliva of German children living in three different areas of traffic density. Sci Total Environ. 2002;297(1-3):109–118. doi: 10.1016/s0048-9697(02)00101-8][Savinov SS, Anisimov AA, Drobyshev AI. Problems and optimization of sampling, storage, and sample preparation in the determination of the trace element composition of human saliva. Journal of Analytical Chemistry. 2016;71(10):1016–11021. (In Russ).][Bulkina NV, Borodulin VB, Osipova YuL, et al. Biochemical changes in saliva of patients with chronic generalized parodontitis under combined action of alternating running magnetic field and laser radiation. Saratov Journal of Medical Scientific Research. 2009;5(3):390–393. (In Russ).]