Serum MicroRNAs as Predictors of Diagnosis and Drug-resistance in Temporal Lobe Epilepsy: A Preliminary Study


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Abstract

Objective:Temporal lobe epilepsy (TLE) is the most common form of refractory focal epilepsy, and the current clinical diagnosis is based on EEG, clinical neurological history and neuroimaging findings.

Methods:So far, there are no blood-based molecular biomarkers of TLE to support clinical diagnosis, despite the pathogenic mechanisms underlying TLE involving defects in the regulation of gene expression. MicroRNAs (miRNAs) have emerged as important post-transcriptional regulators of gene expression.

Results:Recent studies show the feasibility of detecting miRNAs in body fluids; circulating miRNAs have emerged as potential clinical biomarkers in epilepsy, although the TLE miRNA profile needs to be addressed. Here, we analysed the diagnostic potential of 8 circulating miRNAs in sera of 52 TLE patients and 40 age- and sex-matched donor controls by RT-qPCR analyses.

Conclusion:We found that miR-34a-5p, -106b-5p, -130a-3p, -146a-5p, and -19a-3p are differently expressed in TLE compared to control subjects, suggesting a diagnostic role. Furthermore, we found that miR-34a-5p, -106b-5p, -146a-5p and miR-451a could become prognostic biomarkers, being differentially expressed between drug-resistant and drug-responsive TLE subjects. Therefore, serum miRNAs are diagnostic and drug-resistance predictive molecules of TLE.

About the authors

Gloria Bertoli

Institute of Molecular Bioimaging and Physiology, National Research Council (IBFM-CNR)

Author for correspondence.
Email: info@benthamscience.net

Francesco Fortunato

Institute of Neurology, Department of Medical and Surgical Sciences, University "Magna Graecia"

Email: info@benthamscience.net

Claudia Cava

Institute of Molecular Bioimaging and Physiology,, National Research Council (IBFM-CNR)

Email: info@benthamscience.net

Ida Manna

Section of Germaneto, IBFM-CNR

Author for correspondence.
Email: info@benthamscience.net

Francesca Gallivanone

Institute of Molecular Bioimaging and Physiology,, National Research Council (IBFM-CNR)

Email: info@benthamscience.net

Angelo Labate

Neurophysiopatology and Movement Disorders Clinic, University of Messina

Email: info@benthamscience.net

Antonella Panio

Institute of Molecular Bioimaging and Physiology,, National Research Council (IBFM-CNR)

Email: info@benthamscience.net

Danilo Porro

Institute of Molecular Bioimaging and Physiology, National Research Council (IBFM-CNR)

Email: info@benthamscience.net

Antonio Gambardella

Institute of Molecular Bioimaging and Physiology,, National Research Council (IBFM-CNR)

Author for correspondence.
Email: info@benthamscience.net

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