Study of the remote effect of a new technical product — benzoyl urea derivative class

Introduction. The study of the remote effects (embryotoxicity, teratogenicity and reproductive toxicity) of new technical products on the mammalian body is the scientific basis for the safe use of pesticides.

The purpose of the study is to study the effect of a new technical product of the benzoyl urea class on the development of remote effects in warm-blooded animals (rats).

Material and methods. The evaluation of embryotoxic and teratogenic effects was carried out on laboratory animals (rats, males and females). Doses were studied: 0, 1/5000, 1/2500 and 1/1250 LD50. Each group of females consisted of 15 animals with a body weight of 230–240 g at the beginning of the study. The technical product was administered orally to female rats daily during 6–20 days of pregnancy. Fertilization of females with intact males was carried out in a ratio of 2 : 1. Reproductive toxicity was studied on two generations of rats (males; 10 individuals per group and females; 20 individuals per group) at doses of 0, 1/5000, 1/2500 and 1/624 LD₅₀.

Results. Embryotoxic and teratogenic effects were not detected, significant changes in reproductive toxicity were established at the maximum dose (for parents and offspring).

Limitations. In study, there are no data on the study of organ tissues, nor have the processes of cell damage as a result of exposure to a new technical product derived from the benzoyl urea class been studied.

Conclusion. The conducted studies have confirmed that the studied product of the benzoyl urea class belongs to low-risk compounds in terms of embryotoxicity and teratogenicity; moderately dangerous in terms of its effect on the reproductive function of warm-blooded (rats).

Compliance with ethical standards. Compliance with ethical standards: The conduct of the study was approved by the commission of the Ethics Committee of the FSB FNTSG named after F.F. Erisman of Rospotrebnadzor (protocol of the meetings No. 4 at 14.03.2019).

Contribution of the authors:
Rakitskii V.N. — concept and design of the study, editing.
Chkhvirkia E.G. — writing a text, compiling a list of references, editing.
Epishina T.M. — collecting and processing material, writing text, compiling a list of references, statistical data processing.
All authors have made an equivalent contribution to the preparation of the publication (33.3%), are responsible for the integrity of all parts of the manuscript and the approval of the final version of the manuscript.

Acknowledgment. The study had no sponsorship.

Conflict of interest. The authors declare no conflict of interest.

Received: April 26, 2021
Accepted: August 10, 2021
Published: June 28, 2022