Vol 24, No 12 (2017)

Increase of electromagnetic radiations level in the human environment is connected with the accelerated rates of information and communication development. At the same time, ability of peripheral blood lymphocytes of the irradiated persons to the adaptive response in long date after radiation allows to consider them as a biological marker of a functional status of these cells. The quantity of DNA single-strand breaks in peripheral blood lymphocytes affected by various physical factors: the microwave oven - gamma and laser radiation, and after lymphocytes incubating in the environment containing silver nanoparticles was defined by means of fluorescent spectroscopy. It is shown that with increase in microwave radiation frequency DNA quantity in lymphocytes increases in comparison with control samples: under the influence of radiation with a frequency of 3,5 GHz at 32,3 ± 0,9 %, with a frequency of 50 GHz at 40,1 ± 1,1 %, with a frequency of 70 GHz at 49,8 ± 0,7 %. Affection of 137Cs gamma rays preparation with 0,104 MBK activity induces dose-related increase of DNA single-strand breaks. Quantity increase of DNA single-strand breaks is observed after laser irradiation at the wavelength of 510,6 nm to 18,1 ± 0,7 % (irradiation time - 3 min) and to 6,1 ± 0,5 % (irradiation time -5 min), at the wavelength of 578,2 nm to 18,1 ± 0,7 % (irradiation time - 3 min) and to 22,3 ± 0,9 % (irradiation time - 5 min). DNA single-strand breaks quantity measurements after lymphocytes incubation in the normal saline containing silver nanoparticles showed that in the studied range of silver nanoparticles concentration with the diameter of 12 nm ± 10%(1,863 - 0,621 mkg/l) occur structural failure of DNA molecules. On the basis of the obtained data the conclusion has been made that DNA single-strand breaks count in the immune competent cells can become a tool for research of physical factors influence on a human body.

The article presents the research results of a regulatory profile of cardiac rhythm in a situation of examination, depending on the level of personal and state anxiety. In order to identify the characteristics of a psychosomatic response to the situation of examination stress the level of anxiety was assessed, which made the basis for ranking groups (low-, moderate-, high - anxious cadets). Cardiac rhythm variability was investigated by the method of R. M. Baevsky, and the statistical and spectral analysis was estimated. The study involved 42 apparently healthy cadets of the first course, 35 girls and 7 boys. The average age was (17.5 ± 0.5) years. A cross-sectional (transverse) study was carried out which protocol included 2 stages: the first stage: anxiety level was determined before the exam, functioning parameters of cardiovascular system and cardiac rhythm variability were registered. The second stage: registration of above mentioned parameters immediately after the examination. The analysis of the research results showed that the level of state anxiety before the exam in all groups was above the level of trait anxiety, which was seen as a natural reaction to the situation of the examination. The reaction of the cardiovascular system and the mechanisms of the autonomic regulation of heart rhythm in a standard situation such as examination were provocative depending on the level of anxiety. Highly anxious examinees showed a higher activation of sympathetic part of autonomic division of nervous system which indicated the generalization of anxiety and possibilities of its somatization.

The results of biochemical parameters evaluation of blood dynamics and large intestine endoecology in patients with a syndrome of dependence on alcohol are presented. Friendly changes in enzymatic activity of blood serum and quantitative content of the main microflora representatives of large intestine in patients with acute alcoholic psychosis (AAP) are identified. It was produced ranking indicators of serum enzymes, lipids, pigment metabolism with subsequent calculation of the deviations from the physiological norm limits. The total degree of deviation indicators was considered as independent variables, the relationship that reflects a comprehensive assessment of the functional state of the liver to the extent that the large intestine dysbiosis. A mathematical model of regression-type complex quantitative estimation of biochemical status and microbiocenosis rectum at the AAP, based on the scale level, which reflects the cumulative severity of the patient's existing endoecological violations in the values of the analyzed parameters is made. Dynamics assessment of analyzed indicators changes in the course of treatment has allowed to identify the most important biochemical and microbiological parameters for future inclusion as predictors of liver disease progression risk in the development in a mathematical model of the relationship reducing the effectiveness of liver detoxification and dysbiotic shifts the large intestine microflora. The proposed approach can be used to facilitate the evaluation of the recovery of liver function in the process of the treatment on the level of the share of the mutual influence of the recovery in the intestinal microbiocenosis quantitative variables.

Migration of leukocytes through the endothelium of microcirculature is regulated by adhesion molecules. Taking into account the important role of leukocytes migration processes into the skin of patients with psoriasis information about the concentration of adhesion molecules on neutrophils, monocytes and lymphocytes considering their morphological features has not only theoretical but also practical interest, because regulation of this process gives new possibilities for therapy. The study of leukocytes migration mechanisms is necessary for predicting the course of the disease and justifying the search for new effective methods of treatment. The purpose of this research was to investigate the migration of leukocytes carrying adhesion molecules from the peripheral blood to the skin in psoriasis and their role in the development of the pathological process. The clinical and immunological examination of 82 patients with vulgar and exudative psoriasis in the progressive and inpatient stage (39 women and 43 men) aged 20 to 60 years was carried out. Duration of the disease was from 3 months to 10 years. As a control group, 50 practically healthy people were examined (28 women and 22 men). Microscopic venous blood smear was carried out, the segmentogram of neutrophils, lymphocytograms, monocytes were analyzed and at the same time the content of leukocytes expressing receptors for adhesion molecules was determined. Under conditions of hyperproliferation of keratinocytes, as observed in psoriasis, a decrease in the number of neutrophils carrying L-selectin molecules, ICAM-1, LFA-1, LFA-3, PECAM-1 was registered. The level of lymphocytes with the molecules of ICAM-1, LFA-3 slightly increased, but a number of lymphocytes with molecules of L-selectin, LFA-1, PECAM-1 decreased. Changes in the level of monocytes carrying adhesion molecules were absent. The results of the statistical analysis allowed assessing the relationship of peripheral blood leukocytes carrying adhesion molecules, with their morphological characteristics in patients with psoriasis.

Objective. In article describes two original algorithms for the analysis of video-oculographic data and analyses the effectiveness of these algorithms to assess the current functional condition of a person. One of the algorithms is designed for estimating macrosaccades curvature and the other - to evaluate the smoothness of target tracking. Both algorithms are based on geometric methods of videooculographic data processing. Methods. The assess of the algorithms effectiveness was realized on the model of alcohol intoxication (used the medium doses of alcohol - 0.8 g of 96 % alcohol per 1 kg of body weight). For the simulation of saccadic movements and smooth tracking we developed two psychomotor tests, which were evaluated by two key indicators: the curvature of microsaccade and smooth target tracking. Results. The results showed that the operator's activity disorders were usually accompanied by disturbance of the smooth oculomotor tracking. However the significant changes in the curvature of macrosaccades were not observed. Conclusions. Indicators of the smooth oculomotor tracking turned out to be quite informative for assessing the functional state of a person during the activity and can be used for practical diagnosis. Indicators of curvature of microsaccade were not sufficiently sensitive to the negative external factors and can't be used for practical diagnosis of the current condition of the person.